Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing [RNA-seq, transdifferentiation]

We examine the differential transcriptional dynamics during the C/EBPa-driven transdifferentiation of B-cells into iMacs in cells with experimentally induced hypermethylation of the IL1RN gene promoter. We conducted RNA-seq experiments to examine how gene expression changes in cells that underwent hypermethylation editing at the IL1RN promoter using CRISPR-dCas9-DNMT3A during transdifferentiation. We compared the edited cells (sgIL1RN) to non-edited cells (CTRL) at three different time points (0 days -B cells-, 3 days, and 7 days -iMacs-) using biological duplicates.