Comparison of the Helios wide bore and narrow bore injectors in multi-site mass cytometry studies on PBMC samples

To evaluate data quality and inter-instrument variability using the Helios wide bore injector compared to the narrow bore injector in multi-site mass cytometry studies.
Conclusion:
The modified WB+CAS acquisition protocol reduces inter-instrument variability and improves data quality resulting in more comparable data across instruments in multi-site mass cytometry studies,
Notes:
Relative performance improvements seen with the WB injector compared to the NB injector are both somewhat instrument and sample specific. Some Helios instruments may see a much larger change in performance and data quality than others. We prepared a large batch of cells comprised of a pool of CD45 barcoded PBMCs from 5 independent donors stained with the same immunophenotyping panel. Aliquots of these stained cells were distributed to 7 institutions and acquired on 10 different Helios instruments using a shared staining and sample acquisition protocol.