Morphological and molecular identification of fungal isolates, compared with the 454 reads databases.

The morphological identification of the twelve dominant species isolated from the four serpentine substrates was complemented by ITS1-5.8S-ITS2 region sequencing and BlastN analysis (second column). Each sequence was used as a query against the 454 datasets (both ITS1 and ITS2) and the lowest rank identification of the corresponding nearest matching OTU is reported in the third and sixth columns (for ITS1 and ITS2 respectively), together with the bp matching (N of identical bp/bp total lenght of the matching fragment) and the number of sequences supporting the OTU. The last column indicates whether or not (+ or −) the species was detected by both the dilution plate technique and 454 pyrosequencing from the same substrate.