mRNA-seq analysis of Caenorhabditis elegans wild type N2, daf-16(mu86), daf-18(ok480) and daf-16(mu86); daf-18(ok480) starved L1 larvae

Embryos of wild type N2, daf-16(mu86), daf-18(ok480) and daf-16(mu86); daf-18(ok480) were collected by hypochlorite-treat gravid adult worms and were put in virgin S-basal with 0.1% EtOH at 20 °C. After hatching, those embryos entered L1 arrest. Arrested L1s were collected 16 hr post hypochlorite treatment (about 4 hr of starvation). Overall design: Three biological replicates were performed for each of wild type N2, daf-16(mu86), daf-18(ok480) and daf-16(mu86); daf-18(ok480). Arrested L1s were collected after ~4 hr of starvation in virgin S-basal with 0.1% EtOH at 20 °C.