Intact polar diacylglycerol (IP-DAG) lipids measured in cultures of four Antarctic diatom isolates@en

Diatoms were isolated by Adrian Marchetti (http://orcid.org/0000-0003-4608-4775) from waters of the Bellingshausen Sea and grown on replete media. Cells were collected onto 0.7 µm GF/F filters. Extraction was performed using a modified Bligh and Dyer (Bligh and Dyer, 1959; https://doi.org/10.1139/o59-099) method described in Popendorf et al. (2013; https://doi.org/10.1007/s11745-012-3748-0). Lipid extracts were analyzed by HPLC-ESI-MS with data dependent-MS2 acquisition on a high-resolution, accurate mass Thermo Q Exactive Hybrid Quadrupole-Orbitrap mass spectrometer (ThermoFisher Scientific, Waltham, MA, USA) coupled to an Agilent 1200 HPLC system (Agilent, Santa Clara, CA, USA). The HPLC-ESI-MS method is described in Collins et al., 2016 (https://doi.org/10.1021/acs.analchem.6b01260). […]