p53 and p53-downstream targets, CCO activity, mtDNA copy number and deletions in <i>Pten</i> haplo-insufficient mice aged 20–29 weeks.

All outcomes were expressed as percentages of HET values. The values for the outcomes from HET mice were the following: Hippocampus: mtDNA copy number 1214±178; mtDNA gene ratios for ND4, CYTB and COX3 = 0.86±0.05, 1.22±0.02 and 1.65±0.3, respectively; CCO 235±26 and CS 144±14 nmol×(min×mg protein)−1. Cortex: mtDNA copy number 1563±177; mtDNA gene ratios for ND4, CYTB and COX3 = 0.92±0.06, 1.12±0.01 and 1.5±0.2, respectively; CCO 245±19 and CS 170±9 nmol×(min×mg protein)−1. Cerebellum: mtDNA copy number 2600±17; mitochondrial gene ratios for ND4, CYTB and COX3 = 0.83±0.06, 1.02±0.002 and 1.1±0.1, respectively; CCO 60±6 and CS 150±4 nmol×(min×mg protein)−1. (a)Percentage of mtDNA deletions were calculated as follows for each of the three tissues: 100−(100×average of mitochondrial gene ratio HET-CRE/HET). (b)Protein expression was performed by western blot and by normalizing the intensity of the band of the loading control (actin) and expressed as percentage of control values. *p<0.05; **p<0.01; ***p<0.001. Representative western blot images and densitometry results are shown in Figures S3 and S4.