PAPE - Pre-fractionation assisted phosphoprotein enrichment, a novel approach for phosphoproteomic analysis of green tissues from plants

Biological replicates: 393-395 (crude extract): protein extraction 399-401 (40% AS): protein extraction+40% AS precipitation 405-407 (crude extract+MOAC): protein extraction+phosphoprotein enrichment by MOAC 408-410 (40% AS+MOAC): protein extraction+40% AS precipitation+phosphoprotein enrichment by MOAC -determination of protein concentration by 2D-Quant Kit -LC-MS with LTQ Orbitrap Velos: nanoLC, precursor scan Orbitrap, fragmentation CID, fragment scan LIT -Thermo Proteome Discoverer 1.3 with phosphoRS 1.0 -Mascot 2.3 -database TAIR 10 -Scaffold 4 Technical replicates two measurements each (e.g. 393, 393_2)