Effect of PME-1 S156A mutant on gene expression of mouse brain

Protein methylation is a reversible post-translational modification commonly occurring on carboxyl groups of leucine, glutamate, and isoprenylated cysteine, or the side-chain nitrogen atoms of arginine, lysine, and histidine residues. Histone methylation is well studied and shown to play a central role in the regulation of epigenetic gene expression. On the other hand, our knowledge of the importance of methylation in proteins other than histones is limited. Increased PME-1 expression and decreased LCMT-1 expression associated with decreased PP2Ac methylation levels were observed in the brain of AD patients. Therefore, it is crucial to understand the precise role of PP2A methylation in physiological and pathophysiological processes.To obtain a comprehensive view for the effect of loss of PME-1 methylesterase activity on brain development, we performed transcriptome analysis for E18.5 brain tissues. Whole brain of E18.5 mouse brain,PME-1 WT and PME-1 S156A, was collected and then RNA was extracted with TRIzol Reagent for RNA seq.