Metabolic maturation of neonatal hearts driven by ?-linolenic acid in maternal milk [ATAC-seq]

The goal of this study was to compare the epigenetic landscape of perinatal hearts of cardiomyocyte-specific RXRab-deficient (edKO) and WT mice, to identify differential chromatin dynamics that are controlled by TF RXRa and RXRb. Overall design: The chromatin accessibility profiles of WT and edKO perinatal hearts mice were generated using individual P0 hearts as individual biological replicates. Transposition was performed using Tn5 enzyme (Illumina), applying size selection. Sequencing was performed in duplicate using 2×50 HiSeq 3000 (Illumina) and with an average of 25 million paired-end reads per sample.