Thermodynamic parameters from urea denaturation.
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The urea-induced unfolding was analyzed in 20 mM po4 buffer pH7.5, assuming ΔG has a linear dependence on the urea concentration: ΔG = ΔG(H2O)-m[urea], where ΔG(H2O) is an estimate of the value of ΔG in the absence of urea and m is a measure of the dependence of ΔG on the urea concentration. The values for each data point are averages calculated on the basis of at least four independent experiments. Errors shown are derived from the curve-fitting calculations.
创建时间：
2015-12-02
