Single cell transcriptomics of blood mononuclear cells in patients with preeclampsia

Study of single cell transcriptomics of PBMC from patients with preeclampsia and normal pregnancy The study compared changes occurring at the transcriptome level in umbilical cord blood PBMС during normal pregnancy and preeclampsia. During a cesarean section, after the placenta was removed, the umbilical vessel was punctured and blood was collected into EDTA tubes. Isolation of PBMC was carried out in a Ficoll density gradient for 20 min at 800 g with break 0. The PBMС ring was rinsed in PBS and frozen. The samples were then analyzed using single cell transcriptomic analysis