Single-cell RNA-seq uncovers the early alterations of retina in type 2 diabetes mice

Diabetic retinopathy (DR) is a complex disease, the early alterations of which remains to be investigated. We isolated retinas from the control and type 2 diabetes (T2D) mice and conducted scRNA-seq analysis using the 10x Genomics platform. We delineated the complete cellular landscape of retina and explored the early alterations of different cell subpopulations of them in T2D. The Lepr knockout mouse model was used. The retinas were isolated from two control (BKS_WT; m/m) and two type 2 diabetes (BKS_DB; db/db) mice at ages of 13 weeks. The isolated retinas were enzymatically digested to generate single-cell suspensions for scRNA-seq.糖尿病视网膜病变（Diabetic retinopathy, DR）是一类复杂疾病，其早期病理改变仍有待深入探究。本研究从对照小鼠与2型糖尿病（Type 2 diabetes, T2D）小鼠体内分离视网膜组织，并采用10x Genomics平台开展单细胞RNA测序（single-cell RNA sequencing, scRNA-seq）分析。我们绘制了视网膜完整的细胞图谱，并探究了2型糖尿病状态下视网膜不同细胞亚群的早期病变特征。本研究使用了瘦素受体（Lepr）敲除小鼠模型，实验所用视网膜组织取自13周龄的2只对照小鼠（BKS_WT; m/m）与2只2型糖尿病模型小鼠（BKS_DB; db/db）。将分离得到的视网膜组织进行酶解，以制备单细胞悬液用于后续scRNA-seq实验。