Venom on Ice

Accompanying excel spreadsheet details the DNA samples taken and venom glands obtained and associated date/location/depth collection site data.\n\nEach specimen will be DNA barcoded using the diagnostic 600bp region of the mitochondrial COI gene and sequences lodged on GenBank. DNA barcoding will aid in evaluating the extent of cephalopod biodiversity including revealing cryptic species and phylogeographic structure. Confirmation of species identities is also essential as glands from multiple individuals will be required for detailed proteomic analysis of the proteins produced by the octopus posterior salivary glands. The chemical composition of the secretions will be determined through the application of multidisciplinary techniques, including cDNA library construction, mass spectrometry, molecular modelling and bioactivity testing. The studies will commence in 2008 and run for several years, being the major element in a PhD project run through Dr. Fry's laboratory.\n\nData_Format \nExcel file with the following columns:\nSample ID\nDiagnosis\nDescription\nOther Ref\nGland Storage\nTissue Tissue Storage\nVoucher\nPreservation\nImages\nOrder\nFamily\nGenus\nSpecies\nLocation\nSite\nDepth (start-stop)\nLatitude\nLongitude\nDate\nTime (UT)\nCapture\nEvent\nOther Information