Long-read RNA-seq(Iso-seq) analysis of HBV-KMT2B integrated HPCs in which HBV genome is integrated into KMT2B region.

We identified full-length complementary DNA by long-read RNA-seq (Iso-seq) in HBV-KMT2B integrated HCC sample. To compare the data of HCC sapmle with HBV-KMT2B integrated HPCs, which reproduces the sequence of the HCC sample, we performed long-read RNA-seq in HBV-KMT2B integrated HPCs, as well. For Iso-seq, we extracted total RNA from HBV-KMT2B integrated HPCs after removing MEF using Feeder Removal Microbeads mouse (Miltenyi Biotec).