Mechanism of lateral cell-wall expansion at a constant diameter in Bacillus subtilis
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Localization of newly synthesized peptidoglycan in Bacillus subtilis cells. Fields of B. subtilis strain 168 observed by fluorescence (AF532), phase contrast (PC) or bright field (BF) microscopy. Cells in exponential growth phase were grown for 15 min in the presence of ethinyl-D-Ala-D-Ala or in the absence of the probe, fixed, and incubated with azido-AF532 for fluorescent labeling by copper-catalyzed click chemistry.
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2025-05-22



