ProjecTILs human reference atlas of CD8+ tumor-infiltrating T cells, generated by semi-supervised STACAS integration of scRNA-seq datasets spanning 7 cancer types. The dataset is available as a S
We aimed to investigate the association between HPK1 expression and exhausted status of tumor. Overall design: Bulk RNA-seq was performed on fresh-frozen tissue samples of renal cell carcinoma.
CT26 tumors were implanted subcutaneously into syngeneic BALB/C mice and allowed to grow for 15-25 days. Tumors were collected, mechanically dissociated, and immune cells enriched using Percoll gradie
We utilized cell surface expression of CD39 to demonstrate that this marker enriches for CD8+ T cells with features of exhaustion, proliferation and tumor reaxtivity. Overall design: We sorted CD3+ T
