ZNF200 interacts with DDX17 in the RBP-J promoter region to regulate the transcriptional activity of RBP-J.[ATAC-Seq]

We respectively knocked down ZNF200 and DDX17 in the H520 and A549 cell lines. Since H3K4me3 is an epigenetic modification that marks the active state of gene promoter regions and is typically associated with the activation of gene expression, we subsequently performed ATAC-seq experiments using antibodies against histone H3K4me3. Overall design: Knockdown of ZNF200 and DDX17 in H520 and A549 cell lines, followed by ATAC-seq using an H3K4me3 antibody compared to the control group.