Identification of mutants defective for colonization in liver.

Shown are gene insertions that were ≥2.5 s.d. depleted from the pool relative to the mean, based on number of sequencing reads (Experimental Procedures). Library: library harboring the mutations in noted gene; Output/Input: average ratio of the relative abundance of clones containing transposon insertions in gene in the Output liver sample/Input liver sample (± sd); 37/26: average ratio of the relative abundance of clones containing transposon insertions in gene after growth in broth at 37°/growth in broth at 26°C (± s.d.). Data are separated into categories of known virulence factors, amino acid and purine synthesis, LPS modification, and novel candidate virulence factors.