wild type and aire -/- murine meduallary thymic epithelial cells
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE85
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资源简介：
Mice used were B6/129 F2's, 3-5 weeks of age, either wild type or with both copies of the autoimmune regulator gene (aire, GenBank #AF079536) disrupted. Thymi from five of these mice of both sexes were removed and pooled. After collagenase/dispase digestion, density gradient fractionation, and fluorescent antibody staining, cells with the phenotype CD45-, G8.8+, CDR1int and B7.1hi were FACS-sorted and total RNA was made from them. RNA was twice-amplified using a T7 polymerase-based method. Keywords: ordered
创建时间：
2018-02-18
