Profiling the cytotoxic effects of naled and other pesticides in primary human placental cytotrophoblasts

Placental cytotrophoblasts (CTBs) play critical roles in placentation, including implantation, barrier-function, uterine invasion and maternal endovascular remodeling. Impairment of CTB function is linked with common pregnancy complications. In this context, environmental chemicals can contribute to CTB dysfunction. Evidence suggests that prenatal exposures to pesticides affect the placenta and contribute to pregnancy complications and adverse developmental outcomes. Despite being restricted in the European Union, dimethyl 1,2-dibromo-2,2 dichloroethyl phosphate (naled), a common organophosphate pesticide, is widely used in vector control and agriculture in the United States and abroad. In this study, we investigated the placentotoxic activity of naled in 2nd trimester primary human CTBs. We assessed the cytotoxicity of naled and 67 pesticides using the neutral red lysosomal cellular uptake assay and the lactate dehydrogenase release assay. Naled was one of the most toxic compounds (~15..., , , # Profiling the cytotoxic effects of naled and other pesticides in primary human placental cytotrophoblasts

Dataset DOI: [10.5061/dryad.7wm37pw47](10.5061/dryad.7wm37pw47)

## Description of the data and file structure

Supplemental Table 1. Gestational age of placentas used in this study.                                                      

Supplemental Table 2. Screening panel of test pesticides.     

Supplemental Table 3. Pesticide induced cytotoxicity in primary human placental cytotrophoblasts (CTBs; 24h hours).

Supplemental Table 4. Transcriptomes of naled and DMSO exposed primary human placental cytotrophoblasts (24 hours).

Supplemental Table 5. Genes altered due to naled exposure in primary human placental cytotrophoblasts.                                                                       

Supplemental Table 6. Enriched Gene Ontology (GO) Biological Processes associated with DE genes due to naled exposure of primary human placental cytotrophoblasts.                   ...,