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Open Array Custom Real-time quantitative PCR analysis of mouse colonic mucosa. Open Array Custom Real-time quantitative PCR analysis of mouse colonic mucosa

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NIAID Data Ecosystem2026-03-10 收录
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA497872
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Germ-free wild-type mice (GFWT) were inoculated with the commensal CEC (CECWT) or probiotic Nissle 1917 (NissleWT) E. coli strain. Mono-colonized mice, CECWT and NissleWT, were sacrificed 21 days post-inoculation and the colonic gene expression profiles analyzed by high-throughput qPCR using TaqMan OpenArrays. Overall design: qPCR gene expression profiling. Colonic mucosa from germfree mice were used as control. Equal amount total RNA from each mouse was pooled prior to gene expression analysis.
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2018-10-22
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