Open Array Custom Real-time quantitative PCR analysis of mouse colonic mucosa. Open Array Custom Real-time quantitative PCR analysis of mouse colonic mucosa

Germ-free wild-type mice (GFWT) were inoculated with the commensal CEC (CECWT) or probiotic Nissle 1917 (NissleWT) E. coli strain. Mono-colonized mice, CECWT and NissleWT, were sacrificed 21 days post-inoculation and the colonic gene expression profiles analyzed by high-throughput qPCR using TaqMan OpenArrays. Overall design: qPCR gene expression profiling. Colonic mucosa from germfree mice were used as control. Equal amount total RNA from each mouse was pooled prior to gene expression analysis.