PARP inhibition down-regulates constitutive Tnfα transcription.
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(A) Real time qRT-PCR analysis of mRNA accumulation for Tnfα. Histogram indicates mRNA level of cells treated with PJ34 for 1 h (black), relative to untreated cells (grey, value ~1). Values were normalized with Actβ. (B) Real time qRT-PCR analysis of histone H3 and H4 acetylation of Tnfα promoter region. Histograms indicate acetylation level of cells treated with PJ34 for 1 h (black), relative to untreated cells (grey, value ~1). Values were normalized with an internal region of Actβ. Error bars indicate the standard deviation of data obtained from three independent experiments. NT: control cells, no treatment. *p ≤ 0.05.
创建时间：
2016-02-23
