The fate of surface cell layers of <i>Daucus carota</i> (L.) embryos raised in suspension culture

The ultrastructure and fate of surface cells covering mature somatic embryos of <i>Daucus carota</i> grown in suspension culture were analyzed and new information obtained concerning somatic embryogenesis in these conditions. Our studies showed that during some developmental stages, these embryos were covered irregularly and discontinuously by cells with a typical protodermal phenotype characterized by a cuticle on the outer cell wall. We observed that cells with cuticles were peeled off from the surface of mature embryos. Before peeling off, these cells underwent programmed cell death, which was confirmed by the TdT-mediated dUTP nick end labeling method. Transmission electron microscopy revealed advanced processes of autophagy in these cells.本研究对悬浮培养条件下成熟的胡萝卜（*Daucus carota*）体细胞胚表面细胞的超微结构与细胞命运展开了分析，并针对该培养体系下的体细胞胚发生过程获取了全新研究数据。研究结果显示，在部分发育阶段，此类体细胞胚的表面会被具备典型原表皮表型的细胞不规则且间断性地覆盖，这类细胞的外壁特征为带有角质层。我们观测到，带有角质层的表皮细胞会从成熟体细胞胚的表面脱离。在脱落发生前，这类细胞已启动程序性细胞死亡程序，该结论通过末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TdT-mediated dUTP nick end labeling）法得以证实。透射电子显微镜观测进一步揭示，这类细胞内存在处于进展阶段的细胞自噬过程。

搜集汇总

背景与挑战

背景概述

该数据集聚焦于分析胡萝卜（Daucus carota）体细胞胚胎在悬浮培养条件下表面细胞层的超微结构和命运，揭示了这些细胞在发育过程中经历程序性细胞死亡并脱落的现象。数据集通过透射电子显微镜和TUNEL标记等方法，提供了关于细胞自噬和超微结构变化的详细数据，以支持植物发育生物学和生物技术领域的研究。