CUT&Tag sequencing of human pancreatic acinar and ductal cells

Flow-sorted pancreatic acinar and ductal cells from fresh human pancreatic exocrine tissue were subjected to molecular characterization to identify lineage-specific expression and epigenetic properties. Cells of both lineages were cultured and transformed via lentiviral mutation to form pancreatic ductal adenocarcinoma. Overall design: Flow-sorted pancreatic acinar and ductal cells from fresh human pancreatic exocrine tissue were subjected to CUT&Tag sequencing with antibodies for H3K4me1 and H3K27ac, for differential analysis of epigenetic state by lineage