Distinct N6-methyladenosine (m6A) Regulation of Innate Immune Responses During Bacterial and Viral Infections I

N6-methyladenosine (m6A) is a dynamic post-transcriptional RNA modification influencing all aspects of mRNA biology. Here, we examined cellular m6A epitranscriptomes during infections of herpes simplex virus type 1 (HSV-1) and lipopolysaccharide (LPS) stimulation to identify m6A-regulated innate immune response genes. We showed that a significant portion of cellular genes including many innate immune response genes underwent m6A modifications in 5'UTR and 3'UTR, from which we identified common and distinct m6A-modified genes under different stimulating conditions. Overall design: HeLa cells were infected with HSV1 at 5 MOIs and harvested at 5 or 8 hr post-infection. RAW264.7 cells at 4×105 cells per mL were treated with LPS at 10 ng/mL and harvested at 6 hr post treatment. MeRIP was performed to enrich m6A methylated mRNA with m6A antibody, and sequenced with corresponding inut RNA.