Table 3 in Chemotaxonomic investigation of Apocynaceae for retronecine-type pyrrolizidine alkaloids using HPLC-MS / MS

Table 3 Amino acid motifs of homospermidine synthase-like genes (Livshultz et al., 2018a) from Apocynaceae species tested for PAs. The VXXXD motif is associated with evolution of a functional HSS in comparative analyses of PA-producing angiosperms.
Tribe/Genusspecific epithetHSS-likePA phenotype (Tables 1Commentsubfamilymotifand 2, S2)WrightieaeWrightianatalensisnot detected (120-)No hss -like gene identified in transcriptome. Congeneric species havemetabolites identified as PAs with moderate confidence (Table 2).NerieaeAlafiabarteriIXXXDnot detected (120-)One sample analyzed. Retronecine-core PAs reported in Alafia cf. caudata (Colegate et al., 2016).MalouetieaeGalactophoraschomburgkianaVXXXDmoderate confidence(120 + /138 + /156-)HolarrhenacurtisiiVXXXDlow confidence (120 + /138?/156?)ApocyneaeAnodendronoblongifoliumVXXXDnot detected (120-)Platynecine-core PAs reported in Anodendron affine (Hirata and Sasaki, 1970)AnodendronparviflorumVXXXDnot detected (120-)OdontadenieaeSecondatiadensifloraVXXXDnot detected (120-)3 samples analyzed.MesechiteaeMandevillaboliviensisVXXXDlow confidence (120 + /138-/156-)EchiteaeEchitesumbellatusVXXXDhigh confidence (120 + /Infra-specific variation, intra-individual plasticity in PA detectability.138 + /156 +)EchiteswoodsonianusVXXXDnot detected (120-)One sample analyzed.ParsonsiaalboflavescensVXXXDhigh confidence (120 + /138 + /156 +)ParsonsiaeucalyptophyllaVXXXDlow confidence (120 + /138?/156?)PeriplocoideaeFinlaysoniainsularumIXXXDnot detected (120-)RaphionacmeflanaganiiIXXXDnot detected (120-)MarsdenieaeMarsdeniaglabraIXXXNmoderate confidence(120 + /138 + /156-)AsclepiadeaeAsclepiassyriacaIXXXNnot detected (120-)hss -like pseudogene