The dynamic transmission of positional information in stau-mutants during Drosophila embryogenesis.

It has been suggested that Staufen (Stau) is key in controlling the variability of the posterior boundary of the Hb anterior domain (xHb). However, its underlying mechanism is elusive. Here, we quantified the dynamic 3D expression of segmentation genes in Drosophila embryos. With improved control of measurement errors, we show xHb of stau- mutants reproducibly moves posteriorly by 10% of the embryo length (EL) to the wild type (WT) position in the nuclear cycle (nc) 14, and its variability at short time windows is comparable as that of the WT. Moreover, for stau- mutants, the upstream Bicoid (Bcd) gradients show equivalent relative intensity noise to that of the WT in nc12-nc14, and the downstream Even-skipped (Eve) and cephalic furrow (CF) show the same positional errors as the WT. Our results indicate that threshold-dependent activation and self-organized filtering are not mutually exclusive but could both be implemented in early Drosophila embryogenesis.
Methods
Raw Figure

Images of an immunostained Drosophila embryo of the fly line stauHL54 (w;stauHL54) acquired by a Zeiss Z1 light-sheet microscope (1920*1920 pixels, 16 bits and a pixel size of 0.286 micrometer). The embryo age is 32 minutes during n.c.14. Images were taken with a W Plan Apo 20*/1.0 water immersion objective and with sequential excitation wavelengths of 638, 561, and 405 nm for  Hb, Kr, and DAPI, respectively. Two z-stack of images with 1 micrometer spacing were taken from two opposite sides by rotating the embryos 180 degree.

Result Display

The image analysis results in the process of source code. Three parts including the images after combining, after projection at different angles and Hb profile extracting.