PAM-DOSE assay of Nme1Cas9/Nme2Cas9/NcCas9. Homo sapiens strain:HEK293T
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资源简介：
HEK293T cells were co-transfected with Nme1Cas9/Nme2Cas9/NcCas9, corresponding sgRNA, SaCas9 and its sgRNA, and pmTmG library plasmids (1:1:1:1:1) . PCR is used to amplify the sequences flanking the Cas9 target locus, and products were subjected to deep sequencing . PAM regions without indel within three bases near PAM were extracted. PAMs were counted and used to generate sequence logos.
创建时间：
2022-10-27
