Seurat_sct_slot_with_annotation.RDS

C. auris, an emerging fungal pathogen, preferentially colonizes human skin and causes outbreaks of systemic infections. However, the factors that regulate the pathogenesis of C. auris are still unclear. Using unbiased scRNA-seq profiling of murine skin infected with C. auris, we identified the host immune factors that are potentially involved in the regulation of C. auris in the skin. Furthermore, our in vivo scRNA-seq reveals that C. auris infection upregulates IL-1Ra in the myeloid cells and limits neutrophil antifungal activity. In addition, our results suggest that C. auris mannan layer can evade IL-1R signaling mediated skin defense. Collectively, our findings identified the immune and non-immune cells involved in the regulation of C. auris in the skin which could open the door to gain insights into the pathogenesis of this emerging fungal pathogen.