BLyS decoy receptor decreases B cell engraftment in NOD <i>rag1^−/− Prf1^−/−</i> mice.

In two experiments, NOD rag1−/− Prf1−/− mice were given 20×106 PBL were injected i.p. for 7 days with 10 ug/day/mouse of huBLyS, then divided into 3 groups of 3 mice each, the 3 groups received either PBS, 10 ug/day/mouse of huBLyS or 10 ug/day/mouse of TACI-Ig for 7 more days. Animals were sacrificed on day 21 and spleens analyzed by immunohistochemistry. Percentage of B cell engraftment was determined by analysis of the percentage of splenic sections staining with anti-CD20 and by FACS analysis of collagenase disrupted spleen samples.