Diel Multi Omics from the Hawaii Diel Sampling (HaDS)

Diel Multi Omics From the Hawaii Diel Sampling (HaDS) Here, we present data from the Hawaiʻi Diel Sampling (HaDS) survey, which represents a high-resolution sampling of surface ocean microbial communities every 1.5 hours for 48 hours across two environments: Kāneʻohe Bay on the windward coast of Oʻahu, Hawaiʻi, and the adjacent offshore. At both the coastal Kāneʻohe Bay station (HP1) and the adjacent offshore station (STO1), we sampled at 33 time-points across 48 hours in August 2021, and subsequently produced 59 metatranscriptomes, 65 short-read metagenomes, 8 long-read metagenomes, and 66 transfer RNA transcript libraries, in addition to measurements of ocean biogeochemistry. We also generated four deeply-sequenced short-read metagenomes from samples collected in the late fall and spring prior to HaDS through routine Kāneʻohe Bay Time-series sampling (Tucker et al., 2021; Tucker et al., 2025). The goal of HaDS is to characterize microbial responses to diel changes in ocean biogeochemistry and investigate mechanisms of transcriptional and translation regulation. These data were collected by Dr. A. Murat Eren of the Helmholtz Institute for Functional Marine Biodiversity. Further information can be found here: https://merenlab.org/data/hads/ and in the forecoming data description paper (Tucker et al., in review). Timestamp is in UTC.