Method for in vitro cyclic preservation of Potato Germplasm

The invention discloses a method for in vitro cyclic preservation of potato germplasm， which includes the following steps: inoculating virus-free potato germplasm seedlings on a preservation medium in a culture container and cultivating under normal temperature and light for 30 days; adjusting the temperature to 15°C-19°C， adjust the light time to 8h， cultivate for 60 days to form test-tube potatoes; continue to cultivate for 300-400 days; then adjust the temperature to room temperature and cultivate for 100-200 days， the virus-free seedlings wither， and the test-tube potatoes develop seedlings; cut off the small seedlings or transfer the tube potatoes directly to the preservation medium and continue to preserve. The invention provides a method for in vitro cyclic preservation of potato germplasm. Its beneficial effects are: no hormones or plant growth regulators are added in the preservation process， which can avoid genetic variation of germplasm; the equipment used in preservation is simple， and it is not used in autumn and winter， the temperature increasing and cooling equipment does not require special refrigeration in spring and summer， which saves energy; the germplasm storage period is long， and the test tube potato pellets produced are large; simple operation， low cost， safe and reliable.