Integrate multi-omics analysis of LINC01767 in HCC

Hepatocellular carcinoma (HCC) is a significant contributor to cancer-related mortality rates worldwide. In previous studies, LINC01767, a type of ncRNAs, has been identified as playing a crucial role in the metastasis of colorectal cancer to the liver and was included in the diagnostic signature of HCC. No study of LINC01767 in HCC was published. We conducted a multi-omics analysis to examine the roles of LINC01767 in HCC at the first time. We employed bioinformatic analysis to evaluate its diagnostic and prognostic performance, as well as its correlation with clinical characteristics. We further used RNA sequencing and bioinformatic analysis to reveal the underlying mechanism, including its sensitivity to drugs. Our findings suggest that LINC01767 is a potential diagnostic marker and a stable independent OS predictor for HCC. It may associate with cancer stem cells in HCC, the RNA-seq and bioinformatic analysis identified its regulatory gene net and the potential sensitive drug including the PD1. The in vitro experiment verified it would influence the growth and clone formation of Huh7 cell. We conducted the RNA sequencing of 6 samples，including 3 control and 3 treatment. The sample was liver cancer cell line Huh7.and the control sample was Huh7 transfected with blank plasimd named NC1,NC2,NC3,while the treatment samples were Huh7 trasfected with LINC01767-overexpression plasimd;,named OE1,OE2,OE3