Genome-wide profiling of mutant p53 unveils a subset of lncRNAs correlated with colorectal cancer stemness maintenance [ChIP-seq]

RNA-sequencing and ChIP-sequencing were used to trace the lncRNAs regulated by p53-R273H in HCT116 endogenous p53 point mutant cell lines. Overall design: We set out to analyze the differentially expressed lncRNAs in p53-R273H colorectal cancer cell lines compared to wildtype control cell lines (wildtype cell lines as reference) by RNA-seq combined with ChIP-seq. RNA-seq profiling contained three replicates including p53-R273H mutant cell lines and wildtype control cell lines respectively. ChIP-seq profiling contained two replicates including input samples and ChIP samples both of p53-R273H mutant cell lines and wildtype control cell lines.