RNA sequencing analysis of organoids and their parental tissues

We assessed whether organoids maintained the gene expression signatures of their parental healthy tissues and tumors. We performed transcriptomic analysis by bulk RNA sequencing (RNA-seq) across 3 mice, 3 patients and different periods iPSCs. Total RNA of organoids and their parental tissues were extracted at day 7 and day 0, respectively. Total RNA of iPSCs were extracted at day 0, day 1 and day 4. The results show that the organoids are able to replicate the inter-patient heterogeneity, which determines their feasibility on personalized therapy evaluation. Overall design: RNA sequencing analysis of 3 mice(lung, liver and kidney organoids and their parental tissues), 3 patients(organoids and their parental tissues) and iPSCs at day 0, day 1 and day 4