Estrogen receptor alpha ChIP-seq in p53 null mouse mammary gland

ERa is a major driver for breast cancer initiation and progression.However,the fundamental mechanisms,including global cistromic and genomic transcriptional responses that are required to elicit breast cancer initiation and progression in response to ERa, have not been elucidated. We used chromatin immunoprecipitation followed by deep sequencing (ChIP-seq) to identify estrogen regulated genes that directly recruit ERa in the p53 null mouse mammary gland Overall design: Two replicate ERa ChIP samples and one input DNA samples from p53 null mouse mammary glands after mice are treated subcutaneously with 17ß Estradiol for 8 hours.