AAV-mediated Inner ear gene delivery triggers mild host immune responses in the mammalian inner ear

Hearing loss is a common disability affecting the world’s population. However, its treatment options are limited. More recently, adeno-associated virus (AAV)-mediated inner ear gene therapy has shown great promise as a treatment for hereditary hearing loss. However, the host immune responses to AAV-mediated gene therapy in the mammalian inner ear are not well understood. In this study, two serotypes of AAV vectors were injected individually into the mouse inner ear to evaluate the host innate and adaptive immune responses up to one month after inner ear gene delivery. Our results suggest that the host innate and adaptive immune responses to AAV-mediated inner ear gene delivery are limited and mild, which is favorable for its clinical translation., , , # AAV-mediated Inner ear gene delivery triggers mild host immune responses in the mammalian inner ear

Dataset DOI: [10.5061/dryad.9p8cz8wth](10.5061/dryad.9p8cz8wth)

## Description of the data and file structure

This dataset contains raw data collected at the NIDCD/NIH and published under DOI: 10.1016/j.omtm.2025.101456. If you use this dataset, please cite the associated publication as a reference.\"

The datasets contain 10 folders. The names and descriptions are as follows:  

**Figure 1:** Confocal images for localization of resident macrophages in the mouse cochlea. *Cx3cr1^GFP/GFP^* mice are used to localize resident macrophages in the cochlea.  

**Figure 2:** Confocal images for showing chronological changes in macrophage activation after AAV2.7m8-CAG-tdTomato inner ear gene delivery in *Cx3cr1^GFP/GFP^* mice

**Figure 3:** Confocal images for presenting macrophage and T-cell activation after AAV-mediated inner ear gene delivery in C57BL/6 mice ...,