Search for cell lines affected by molecular hydrogen

The molecular mechanisms underlying the effects of hydrogen remain almost unclear. We found that hydrogen enhances proliferation of some of seven human cancer cell lines. Each cells cultured with following normal condition. A549, SH-SY5Y, HepG2, HT1080, PC3, HCT116, and HeLa cells are derived from human cancer cells, and were obtained from Riken BRC. Medium for HepG2, HT1080, HCT116, and Hela cells was “DMEM, high glucose” (11965126, Gibco) with 10% fetal bovine serum (FBS, Gibco), 1% Penicillin-Streptomycin (Gibco), 20 mM HEPES-NaOH pH 7.4. Medium for A549 cells was “MEM” (11095080, Gibco) with 10% FBS, 1% Penicillin-Streptomycin, and 20 mM HEPES-NaOH pH7.4. Medium for SH-SY5Y cells was “DMEM, high glucose, no glutamine” (11960, Gibco) with 10% FBS, 1% PS, 2 mM L-glutamine (Nalgene), and 1% Non-Essential Amino Acids for MEM Eagle (MP Biomedical). Cells were cultured in a 37°C humidified incubator with 5% CO2.