This article investigates the impact of N-terminal sequences on translation efficiency during protein synthesis. By constructing and screening a large library of eGFP variants, the authors discovered
Bacterial cDNA expression libraries are made to reproduce protein sequences present in the mRNA source tissue. However, there is no control over which frame of the cDNA is translated, because translat
There is a quest for production of soluble protein of high quality for the study of T-cell receptors (TCRs), but expression often results in low yields of functional molecules. In this study, we used
