Hemodynamic recovery of hearts receiving inhibitors of specific enzymes before IPC.

Hemodynamic parameters were calculated at completion of the reperfusion: for perfusion only, I/R and IPC+I/R. (For experimental protocols see Figure 1 groups 1–3 and 7–9).SnMP- hemodynamic recovery of hearts receiving 2 µmole of the HO inhibitor SnMP.Proteases cocktail- hemodynamic recovery of hearts receiving a cocktail of proteasome and lysosomal proteases inhibitors. Dose 1 of the cocktail was composed of MG132, 6 µmol/L; leupeptin 23.4 µmol/L; pepstatin-A 8.8 µmol/L.Proteasome inhibitor- hemodynamic recovery of hearts receiving 3 µmol/L of the proteasome inhibitor MG132.The numbers in parentheses indicate the number of repetitious experiments. Controls (considered 100%) are the heart’s hemodynamic parameters at the end of a 10 min perfusion period (end of the stabilization period).Results are means±SE.*Significantly different from controls-perfusion only (p