Fc receptor-like 6 (FCRL6) defines pre-BCR dependent and independent pathways of natural antibody selection

Overall Design: FCRL6+ and FCRL6- pro B (AA4.1+Lin-CD43+B220+CD19-IgM-) cells, derived from the fetal livers of embryonic day 18 and bone marrow of adult 8-12 week old mice, were sorted by flow cytometry from BALB/cJ strain mice. Single cell suspensions from leg bones were pooled from 5-10 adult male and female mice and 7-10 fetal livers were pooled from embryonic day 18 fetuses isolated from pregnant mothers according to the timing of vaginal plug formation. Data are from a total of four samples that were isolated in duplicate from independent sorts of each tissue. Each sort yielded two samples per cell type (FCRL6+ and FCRL6- pro B cells).