ATM and IRAK1 orchestrate two distinct mechanisms of NF-kB activation in response to DNA damage

DNA damage in cells induces the expression of inflammatory genes. However, the mechanism by which cells initiate an innate immune response in the presence of DNA lesions blocking transcription remains unknown. Here, we find that genotoxic stresses lead to an acute activation of the transcription factor NF-kB through two distinct pathways, each triggered by different types of DNA lesions and coordinated by either ATM or IRAK1 kinases. ATM stimulates NF-kB in cells with DNA double-strand breaks. In contrast, IRAK1-induced NF-kB signaling occurs in neighboring cells via IL-1a secretion from transcriptionally stressed cells caused by DNA lesions blocking RNA polymerases. Subsequently, both pathways stimulate TRAF6 and the IKK complex to promote NF-kB-mediated inflammatory gene expression. These findings provide an alternative mechanism for damaged cells with impaired transcription to initiate an inflammatory response without relying on their own gene expression, a necessary step that injured cells depend on during canonical innate immune responses.