Expression of SOCS proteins following EBV infection of human monocytes.

Monocytes (5×106) were stimulated or not with EBV for the indicated time. (A) The expression of SOCS1 and SOCS3 proteins was evaluated by RT-PCR using described primers (Table 1) or (B) by Western blot analysis using specific anti-SOCS1 antibodies, anti-SOCS3 antibodies and anti-Actin as loading control. Densitometry was performed and represents fold protein induction (relative to 0 min.) ± std. dev. of experiments performed in duplicate. Data are representative of three independent experiments.