Small molecules that induce proliferation of human iPSC-cardiomyocytes

We selected a set of five compounds with diverse known targets that induce proliferation of human iPSC-derived cardiomyocytes. We performed bulk RNA-sequencing of human iPSC-CMs in response to the five compounds to identify common mechanisms mediating compound-induced cell cycle progression. Transcriptome profiling revealed these compounds all induced a common set of pathways including cell cycle, DNA repair, and kinesins. Cellular Dynamics iCell Cardiomyocytes were treated with DMSO or 1 of 5 small molecules (C1, C2, C3, C4, C5) for 24 hours. Each condition had 3 replicates.