Data from: Cellular and transcriptional trajectories of neural fate specification in sea anemone uncover two modes of adult neurogenesis

This archive contains raw data and code associated to the following publication: Cellular and transcriptional trajectories of neural fate specification in sea anemone uncover two modes of adult neurogenesis Plessier and Marlow, 2026, Nature Communications This archive is available for download here on zenodo Folders content: - _raw/: raw scRNA-Seq data matrix file used as the primary data source - _SeuratObjects/: processed and annotated Seurat objects (global KikGR+ cells clustering, each line-specific reclustering, progenitor-specific reclustering, and cnidocyte-specific reclustering) - _code/: contains the scripts for processing and analysis of scRNA-Seq data - _input/: contains input files required to run the scripts - _output/: output plots generated by the code Sequencing data availability: Raw sequencing reads for the scRNA-Seq dataset are available in the GEO repository GSE288441. These include paired-end fastq files for each of the 84 scRNA-Seq library in the paper, alongside the reference genome from the Darwin Tree of Life Consortium from the Wellcome Sanger Institute Tree of Life programme, NCBI GCF_932526225.1 RefSeq assembly and associated NCBI RefSeq gene models jaNemVect1.1 GTF with the KikGR transgene sequence added to both. These also include cell metadata information and raw and filtered global count tables. Softwares: R (4.4.1) open source packages Seurat (5.2.1), tidyverse (2.0.0), WGCNA (1.73), SeuratObject (5.0.2), reshape2 (1.4.4), ggnetwork (0.5.13), ggraph (2.2.1), ggnetwork (0.5.13), igraph (2.1.4), dynamicTreeCut(1.63-1), stringr (1.5.1), tidyr (1.3.1), dplyr (1.1.4), here (1.0.1), purr (1.0.4), ggplot2 (3.5.1), fgsea (1.30.0), patchwork (1.3.0), writexl (1.5.1). readxl (1.4.5), slingshot (2.12.0), viridis (0.6.5), viridisLite (0.4.2), mgcv(1.9-1), ggtext (0.1.2).