Analysis of human dendritic cells in blood, spleen and skin

Analyze human dendritic cells (DCs) in blood and tissues using mass cytometry. Human blood, spleen and skin were enriched for myeloid cells using negative selection. Samples were stained for mass cytometry using a protocol to detect transcription factors and acquired in a CyTOF2
Conclusion:
In order to characterize dendritic cell heterogeneity using mass cytometry it is necessary to perform a negative selection to enrich for myeloid cells.