Early Secretory Pathway glycoprotein analysis dataset

Protein misfolding is one of the causes for several diseases and as a consequence, analysis of the misfolded protein fraction is one of the major aims in translational research. Here, we report a workflow and its associated dataset which focuses on the analysis of the Early Secretory Pathway (ESP) glycoproteins (gESP) from melanoma cells. Our results provide an overview on the ESP glycoprotein members by analysis of both the peptide and glycan chains. Moreover we provide the quantitative analysis of cells treated with kifunensine (an immunomodulatory agent), which blocks class I mannosidases processing of the misfolded or unfolded glycoproteins sent to degradation. Our dataset provides an overview of the major cargo of ESP glycoproteins, underpinning novel candidates for glycoprotein-related Endoplasmic Reticulum Associated Degradation (ERAD) which could be involved in melanoma antigen presentation.