Supplemental Material for Espinoza and Berg, 2020

Figure S1: Egg chambers develop sequentially in the
ovary. A) Schematic diagram of Drosophila
ovaries depicting egg chambers developing progressively in assembly lines
called ovarioles. Each ovariole contains
egg chambers organized sequentially, with youngest at the anterior and most
mature at the posterior. B) Schematic
diagrams of three stages of egg-chamber development showing somatic follicle
cells in white and germline-derived nurse cells and oocyte in light
orange. Nuclei are represented by blue
circles. Dorsal-appendage-tube lumen is
shown in black in S12. C) Timing of GAL4
expression. CY2-GAL4 is expressed
at stage S6 in all follicle cells, while c415-GAL4 is expressed at stage
S10B only in the stretch cells; in both cases the expression continues to the
end of oogenesis.

Figure S2: Modifier Screen crosses used to identify and
narrow down regions that suppress or enhance the Idgf3-overexpression
phenotype. Chromosome IV is not
shown. A) Cross to obtain the control
females. Females from the Idgf3-overexpression
stock, which was kept at 25o for the main modifier screen and then
kept at 22o for narrowing down regions, were crossed to w1118
males. B) Cross to obtain the testing
females. Females from the same Idgf3-overexpression
stock from A were crossed to males from each stock of the 3L deficiency kit to
obtain the females for testing; these females were identified by the lack of
the Balancer chromosomes (which carry a dominant marker).

Figure S3: Collage of representative eggs observed in the
modifier screen, grouped into three categories: Normal/mild, moderately
defective, and severely defective dorsal appendages (DAs). The DAs on normal eggs have a round paddle
and flat stalk, with each making up ~ 50% of the length of the DA. In addition, the edges of the DAs are
smooth, and the bases do not touch. Mild
eggs had DAs that closely resembled those of normal eggs but had one slightly
defective feature, such as marginally wider paddles or bases that were closer
together. Eggs were classified as
moderately defective when having wavy or serrated DA edges; some eggs had
triangular-shaped DAs or DAs of increased size; some eggs had no clear
separation between paddle and stalk.
Eggs were classified as severely defective when they were half the
length of a normal egg, or when the eggs were of normal length, but the DAs had
chorion material that did not resemble normal DAs. For example, some eggs had chorion material
in between the DAs, or the chorion material meant to form the DAs was in a pool
on the dorsal anterior side of the egg.
Some eggs in this category had DAs in which their stalks were completely
fused.