Data from: Landscape-scale distribution patterns of earthworms inferred from soil DNA

Assessing land-use effect on the diversity of soil biota has long been hampered by difficulties in collecting and identifying soil organisms over large areas. Recently, environmental DNA-based approaches coupled with next-generation sequencing were developed to study soil biodiversity. Here, we optimized a protocol based on soil DNA to examine the effects of land-use on earthworm communities in a mountain landscape. This approach allowed an efficient detection of earthworm diversity and highlighted a significant land-use effect on the distribution patterns of earthworms that was not revealed by a classical survey. Our results show that the soil DNA-based earthworm survey at the landscape-scale improves over previous approaches, and opens a way towards large-scale assessment of soil biodiversity and its drivers.